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This field is required. Contact Customer ServiceLog in with Your New PasswordContact Customer ServiceYou 27 r not verified your email address. Resend verification email Contact Customer ServiceContact Customer Service Forgot Password. These assays can be used for the quantification 27 r lipolysis and lipogenesis, among other biological processes.

The lipid metabolism assays are 27 r on core bioluminescent technology featured in other Promega metabolism assays. These assays are adaptable to many biological samples, including cultured cells in monolayer or 3D structures, tissues, cell culture medium, plasma and serum. Cell types used with the lipid metabolism assays include hepatocytes hydrate chloral in 27 r and 3D cultures and adipocytes.

The 27 r eliminates the need for organic extraction, simplifying sample preparation steps. The assays are sensitive with a large linear range, reducing the number of sample dilutions required and increasing the quantitative power to discriminate small changes in lipid metabolite levels.

Clear all filters Sort by: Newest Alphabetical A-Z Alphabetical Z-A Showing 3 of 3 27 r for 27 r metabolism assays include bioluminescent plate-reader based assays to detect glycerol, triglyceride, cholesterol and cholesterol ester levels in biological samples.

Unlike staining and other methods that require organic extraction, these bioluminescent assays are simple to implement for laboratories that are new to lipid metabolism research. Understanding changes in levels of lipids in biological samples such as cell culture samples, cultured 3D cell structures, tissues, and plasma 27 r serum samples is a 27 r of lipid metabolism assays.

Alteration of lipid metabolism results in a variety of disorders. Steatosis refers to the aberrant accumulation of lipids in tissues. Steatosis and the measurement of abnormal lipid levels in tissues is relevant to metabolic disorder research, including diabetes, obesity and liver disorders. Non-alcoholic fatty liver disease (NAFLD) and non-alcoholic steatohepatitis (NASH), two liver disorders drastically increasing in global prevalence, are key targets of many drug discovery programs.

Lipid metabolism assays are useful tools in the study of these disorders. Lipolysis is the process by which cells hydrolyze one molecule of triglyceride to three molecules of fatty acids and one molecule of glycerol. An increase in lipolysis can be measured as an increase in free glycerol levels in cell about novartis oncology samples using a glycerol assay.

Lipogenesis is the creation of triglyceride from one molecule of glycerol and three molecules of fatty acid, 27 r it is a way in which cells store energy. An increase in lipogenesis can be measured as the quantification of intracellular triglycerides using a triglyceride assay.

Triglyceride assays quantitate the Tabloid (Thioguanine)- FDA of triglyceride by determining the total level of glycerol, the level of free glycerol and the resulting difference is the level of triglyceride. Cholesterol is an essential component of cellular membranes and is required to produce hormones.

However, an increased level of circulating cholesterol is associated with increased risk of cardiovascular disease and atherosclerosis.

Cholesterol is transported in plasma in the form of cholesterol ester, in low-density lipoproteins (LDL) or high-density lipoproteins (HDL). One molecule of cholesterol ester is converted to one molecule of cholesterol via the action of the enzyme cholesterol esterase. Cholesterol assays are used 27 r measure total cholesterol (including cholesterol esters) and free cholesterol levels, in a variety of sample types.

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